Coldetach™
Gentle, Chemical-Free Spheroid Harvesting for Human Pluripotent Stem Cell Research
A cold-responsive, micropatterned culture surface for reproducible, ROCK inhibitor-free 3D spheroid generation.
SPHEROID HARVESTING TECHNOLOGY
Simplify Spheroid Production
No Chemical, No ROCK inhibitor
Coldetach™ is designed to enable gentle, chemical-free detachment of human pluripotent stem cell colonies using a cold temperature-responsive surface. Its unique micropatterned design produces uniform colonies for homogeneous spheroid production under further 3D culture without damaging cell-cell or cell-matrix interactions—eliminating the need for ROCK inhibitors and enzymatic digestion.
Made for translational
stem cell research
Organoid and 3D culture models
Drug discovery and toxicity testing
Disease modeling
with standardized spheroids
Reproducible hiPSC colony detachment
Enzyme and RI-free harvesting workflows
~100%
or more
post-detachment viability
3.3X
more beating cardiac
organoids observed
95%
retention of
pluripotency markers
Results based on peer-reviewed data and internal studies.
How it works
Seed pluripotent stem cells in Coldetach
Grow to confluence at 37 °C
Cool to 4 °C to trigger spontaneous detachment
Collect uniform colonies for downstream use
Ready for chemical-free detachment of pluripotent stem cell colonies?
Try Coldetach to advance your organoid research development.